ACTIVITY 1

pH Tritration Lab

PURPOSE:

In this experiment students will learn to: titrate a sample, properly use and read a pH meter, conduct quantitative analyses, perform titration calculations.

SUMMARY:

This experiment is designed to teach students to use a pH meter to monitor a titration for its endpoint to determine the acidity of solution (pickle juice). In this lab students will titrate pickle juice with sodium hydroxide to determine the amount of acetic acid present.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two to three students.

ring stand          phenolphthalein             sodium hydroxide

buret clamp       glass stirring rod            narrow-mouth 150-mL

95% ethanol      50 mL brine solution       50 mL dill pickle juice

50-mL buret      distilled water                250-mL beaker (for waste)

Chemwipes or other lint-free tissues

250 mL beaker of 0.25 M sodium hydroxide

distilled water in a wash bottle

pH meter (or Vernier pH probe and CBL unit/TI graphics calculator or computer)

 

SAFETY:

The teacher should review the following with all students taking part in this laboratory exercise. All chemicals may be disposed of in accordance with your local laws. Review the MSDS sheets of all chemicals used in this experiment.

1.   Apron, goggles, and gloves should be worn at all time during lab!

2.  Since the students will be using Sodium hydroxide (NaOH) and it is

      caustic, the instructor should review basic safety precautions

      pertaining to the use of these chemicals.    

      If you should spill NaOH on your hands, rinse them with a lot of

      water.

3.  The pH probe is a delicate instrument. The end of the probe

      consists of a thin glass bulb. Because this glass is thin, it can break

      easily. Handle the probe with care and follow your instructor’s

      directions for cleaning. The pH probe can also dry out easily. Be

      sure it is kept in a pH 7 buffer solution when not in use. Do not store

      the probe in water.


 

ACTIVITY 2

Principles of Chemistry Lab

PURPOSE:

In this experiment students will learn to: test organic compound to determine whether they are fats, carbohydrates or proteins, conduct an experiment to determine how heat and cold effect enzyme activity, and construct three-dimensional atomic models of organic and inorganic compounds to observe different types of bonding.

SUMMARY:

In this experiment students will use molecular model kits to build different types of organic and inorganic compounds found in living things. Students will also observe the behavior of enzymes when exposed to heat and cold.  Students will then perform different types of test on organic compounds to determine whether the compound is a carbohydrate, fat or protein.   

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two to three students.

molecular model kit                                tweezers

3% hydrogen peroxide                           milk

4 test tubes                                           Lugol’s or idoine solution

test tube rack                                        Benedict’s solution

thermometer                                          raw egg white

50 mL graduated cylinder                       raw chicken or cow liver starch solution                                         

SAFETY:

The teacher should review the following with all students taking part in this laboratory exercise. All chemicals may be disposed of in accordance with your local laws. Review the MSDS sheets of all chemicals used in this experiment.

1.  Apron, goggles, and gloves should be worn at all time during lab!

2.  Since the students will be using iodine or Lugol’s solution,

     Benedict’s solution and 3% hydrogen peroxide which are all toxic

     And caustic, the instructor should review basic safety precautions

     pertaining to the use of these chemicals.    

     If you should spill any of these solutions on your hands, rinse them

     with a lot of water.

3.  Since a hot water bath will be used to denature the enzyme in the

     liver caution should be observed by students using the heating unit.


ACTIVITY 3

Review of Light Microscope

PURPOSE:

To familiarizes students with the use of a compound light microscope.  Teach students to make slides of prepared human cells to review wet mount and simple slide staining techniques.

SUMMARY:

To provide the students with the opportunity to review the parts of a microscope and proper handling of a compound light microscope. Students will make slides to review wet mount and simple slide staining techniques.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two to three students.

water                                                    forceps

fine newspaper                                      scissors

medicine dropper                                  slide and cover slips

compound microscope drawing              3 basic types of microscope drawing

compound light microscope drawing    

SAFETY:

Review basic care and handling of microscopes.

No special safety consideration is required for this activity.


 

ACTIVITY 4

 Cellular Structure and Function

PURPOSE:

Students observe human cheek cells and their internal organization.  Then they examine the process of cell division and make drawing of major stages.  Students will conduct quantitative analyses to determine the way in which materials cross cell membranes.

SUMMARY:

Students will make cheek cell slides and observe them under a light microscope.  Students will also collect data on what type of materials will cross a membrane.  Students will use a light microscope to examine and draw different stages of cell mitosis. 

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two to three students.

water                                                    scissors

forceps                                                 ring stand

tooth picks                                            thistle tube

dialysis tubing                                       150 mL beakers

copper sulfate                                       400 mL beakers

iodine solution                                       medicine dropper

wood charcoal                                       filter paper funnel

0.1% silver nitrate                                  30% sugar solution

methylene blue stain                              50 mL graduated cylinder

glass slide and cover slips                      potassium permanganate

compound light microscope                    starch-sodium chloride solution

prepared microscope slides of mitosis

SAFETY:

The teacher should review the following with all students taking part in this laboratory exercise. All chemicals may be disposed of in accordance with your local laws. Review the MSDS sheets of all chemicals used in this experiment.

1.  Apron should be worn at all time during lab!

2.  Review basic care and handling of microscopes

3.  Since the students will be using 0.1% silver nitrate, methylene blue   

     stain, and potassium permanganate are all toxic and caustic, the 

     instructor should review basic safety precautions pertaining to the     

     use of these chemicals.

     If you should spill any of these solutions on your hands, rinse them

     with a lot of water.


 

ACTIVITY 5

Extraction of DNA from Cow Thymus

PURPOSE:

Students will extract DNA from a Cow Thymus cells to better understand the amount of DNA each cell contains.

SUMMARY:

Students first help prepare the cow’s thymus so that the cells in this organ will begin to swell and break.  Next using meat tenderizer students and alcohol DNA is taken out of the cells and spun on a pencil 

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two to three students.

cheesecloth                               100 mL water

funnel                                       250 mL beaker

detergent                                  95% ethanol

ice bath                                    pencil

2 cm piece of calf thymus (also called sweetbreads)

SAFETY:

The teacher should review the following with all students taking part in this laboratory exercise. All chemicals may be disposed of in accordance with your local laws. Review the MSDS sheets of all chemicals used in this experiment.

Apron and goggles should be worn at all times during this lab!

ACTIVITY 6

Microscopic Examination of Human Tissue Types

PURPOSE:

Students will examine the structure of the main types of human body tissue.  Students will also identify the function of the various tissues of the body.

SUMMARY:

Students will use a light microscope to examine, classify, and draw the thirteen different major tissue types and found in the human body.  Students will also identify the function of the various tissues of the body.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two to three students.

Prepared slides of each of the following tissues:

Epithelial tissue              Connective/Supportive Muscle                Muscle tissue

1. simple squamous       1. hyaline                                              1. striated

2. stratified squamous    2. elastic                                               2. smooth

3. cuboidal                    3. blood                                                3. cardiac

4. simple columnar         4. bone                                                               

5. stratified columnar     5. adipose

SAFETY:   

Review basic care and handling of microscopes


 

ACTIVITY 7

Integumentary Lab

PURPOSE:

Students will examine the appearance and structure of the skin, hair, scalp, nails, and fingerprints. Students will also collect data on the sense of touch, including heat, pressure, pain, etc. to observe the skins role in communication with the environment.

SUMMARY:

Student examine the skin and make comparison of data collected.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two to three students.

nails                             ink pad             ice bath

compass                       metric ruler       tweezers

microscope                   stiff brush          magnifying glass

fingerprint reference sheet

               

SAFETY:

Care should be taken not to damage skin nor draw blood with compass point.

ACTIVITY 8

Introduction to the Axial Skeletal System

PURPOSE:

Students will learn the names, structure, markings, and location of bones of the axial skeletal system.   Students will also learn internal parts of a long bone. 

SUMMARY:

Students will examine and then label the internal structure of a long bone using real bones and bone models.  Then the students will examine real and plastic models of bones to learn the names, structure, marking, and location of the bones of the axial skeleton system.  Students will also classify the bones they examine as being short, flat, long or irregular.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two to three students.

plastic disarticulating skeleton                 skulls

real articulated skeleton                          plastic encased bones of the ear

SAFETY:

Review proper handling techniques for real bones.

ACTIVITY 9

Introduction to the Appendicular Skeletal System

PURPOSE:

Students will learn the names, structure, markings, and location of bones of the appendicular skeletal system. 

SUMMARY:

Students will examine and then label the internal structure of a long bone using real bones and bone models.  Then the students will examine real and plastic models of bones to learn the names, structure, marking, and location of the bones of the axial skeleton system.  Students will also classify the bones they examine as being short, flat, long or irregular.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two to three students.

plastic disarticulating skeleton     real articulating skeleton

SAFETY:

Review proper handling techniques for real bones.


 

ACTIVITY 10

Introduction to the Muscles

PURPOSE:

To teach students the names, location, origin, and insertions of major muscles of the human body.  This will be accomplished by sculpting muscles onto a plastic human figure.

SUMMARY:

Students will use modeling clay to sculpt the major muscles of the body onto a plastic human mannequin figure.  Students will learn origin and insertions of major muscle groups and their primary action.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two students working together.

modeling clay                mannequin figure           reference text

SAFETY:

Review proper handling of mannequin figure, modeling clay, and use of sculpting instruments.

ACTIVITY 11

Micro Structure of a Muscle

PURPOSE:

Understand relationship between variables.  Develop ability to graph and interpret graphs, find an unknown variable.

SUMMARY:

Students note changes cold and warm water have on handwriting; bicep circumference when flexes and relaxed; gastronemius muscle changes when standing on one’s toes, fatigue effects on finger muscle.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two to three students.

pan of ice                     tape

strips of paper               cloth measure tape

tongue depressor           string & 500 g weight

stopwatch                      sphygmomanometer

SAFETY:

Watch to make sure that students are not risking injury to their fingers.

ACTIVITY 12

Human Reflex Physiology

PURPOSE:

Students will gain a better understanding of how neurons work and reflex nerve responses.

SUMMARY:

Students test the patellar, Achilles, crossed extensor, abdominal, plantar, corneal, gag, papillary light, consensual, ciliospinal, and salivary reflexes.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two students.

reflex hammer                           sharp pencils

cot (if available)                         absorbent cotton

tongue depressor                       metric and 12 in ruler

flashlight                                   wide-range pH paper

100 or 250 mL beaker                large bucket w/ fresh 10% bleach solution

disposable autoclave bag            wash bottle w/ 10 % bleach solution

SAFETY:

Properly dispose of materials


 

ACTIVITY 13

Dissection of a Sheep’s Brain

PURPOSE:

To learn the names, location, and structures of a mammalian brain.

SUMMARY:

Students will dissect a sheep brain to learn general anatomy of the mammalian brain. They will familiarize themselves with the different points of view and plans of section for example dorsal, ventral, lateral and sagittal, coronal, and horizontal planes of section.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two students.

sheep’s brain                            gloves

dissecting tray                           aprons

dissecting kit                             safety goggles

SAFETY:

Preservatives can be dangerous to students that wear contact lenses.  These students, if possible, should wear eyeglasses during lab.

All students must wear gloves and lab aprons during dissection. 

Wearing of safety goggles is also strongly advised during lab.

ACTIVITY 14

Hearing and Equilibrium Lab; Taste and Olfaction Lab

PURPOSE:

To demonstrate knowledge of ear anatomy, test students hearing, and equilibrium.  Students will learn about the sense of smell through data collecting and analysis.

SUMMARY:

Students will dissect a sheep brain to learn general anatomy of the mammalian brain. They will familiarize themselves the different points of view and plans of section for example dorsal, ventral, lateral and sagittal, coronal, and horizontal planes of section.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two to three students.

chalk                Q-tips               cotton               almond

toothpicks         distilled water     oranges            ticking watch

paper towel       paper cups        sugar crystal     tuning forks

peppermint        rotating stool      acetic acid solution

vial of NaCl solution                                     

SAFETY:

Equilibrium subjects should not be easily inclined to dizziness on rotation or turning movements; rotation should be stopped if subject becomes nauseous; classmates should be prepared to catch, hold o9r support subject(s).                                                                                                         

A 10% bleach solution should be used on desk and counter tops in the classroom before beginning the smell lab.

For the sense of smell lab it is important that the students not share anything in this part of the lab.  This includes cups and anything that they have in their mouths.  Students should dispose of their own waste materials.


 

ACTIVITY 15

Dissection of a Cow’s Eye

PURPOSE:

Students will dissect a cow’s eye to learn general anatomy of the mammalian eye. They will familiarize themselves with the different points of view and plans of section for example dorsal, ventral, lateral and sagittal, coronal, and horizontal planes of section.

SUMMARY:

In groups of two students will dissect and examine the different parts of a cow’s eye.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two students.

cow’s eye                     gloves               dissecting tray

aprons                          dissecting kit     safety goggles

SAFETY:

Preservatives can be dangerous to students that wear contact lenses.  These students, if possible, should wear eyeglasses during lab.

All students must wear gloves and lab aprons during dissection. 

Wearing of safety goggles is also strongly advised during lab.

ACTIVITY 16

Lab on Human Vision

PURPOSE:

Determine near point vision, visual acuity, accommodation, astigmatism, and visual mapping.  To gain understanding of human vision.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two to three students.

pins                              cm scale                       astigmatism chart

index cards                   visual map                     peripheral vision cards

Snellen chart

SAFETY:

None

ACTIVITY 17

Forensic Science – Caught By A Kiss

PURPOSE:

This lab is intended to familiarize students with the activity of the enzyme amylase, the primary digestive component of human saliva.  It will also emphasize to students the value of finding saliva at a crime scene, because helpful DNA evidence can be isolated from saliva.

SUMMARY:

This lab allows students to work together using forensic science techniques to determine which of 8 suspects was actually at the scene of  the crime.

EQUIPMENT:

Materials for this laboratory exercise are set up for lab groups consisting of two to three students.

8  toothpicks                              8  straws

8  paper cups                            24 petri plates

8 envelopes                               24 resealable bags

50 absorbent cotton balls            labels

500 mL bottle of Melt-N-Pour starch agar

30mL IKI potassium iodide solution

SAFETY:

Prudent lab safety practices should be followed at all times during this lab.  One portion of the lab requires use of boiling hot liquids. Students should be warned of the danger of getting burned.