ACTIVITY 1
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pH Tritration Lab
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PURPOSE: |
In this experiment students will learn to: titrate a sample, properly
use and read a pH meter, conduct quantitative analyses, perform titration
calculations. |
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SUMMARY: |
This experiment is designed to teach
students to use a pH meter to monitor a titration for its endpoint to
determine the acidity of solution (pickle juice). In this lab students will
titrate pickle juice with sodium hydroxide to determine the amount of acetic
acid present. |
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EQUIPMENT: |
Materials for this laboratory exercise are set up for lab groups
consisting of two to three students. ring stand phenolphthalein sodium hydroxide buret clamp glass
stirring rod narrow-mouth
150-mL 95% ethanol 50
mL brine solution 50 mL dill
pickle juice 50-mL buret distilled water 250-mL beaker (for waste) Chemwipes or other lint-free tissues 250 mL beaker of 0.25 M sodium hydroxide distilled water in a wash bottle pH meter (or Vernier pH probe and CBL unit/TI
graphics calculator or computer) |
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SAFETY: |
The teacher should review the following with all students taking part in this laboratory exercise. All chemicals may be disposed of in accordance with your local laws. Review the MSDS sheets of all chemicals used in this experiment. 1. Apron, goggles, and gloves should be worn at all time during lab! 2. Since the students will be using Sodium hydroxide (NaOH) and it is caustic, the instructor should review basic safety precautions pertaining to the use of these chemicals. If you should spill NaOH on your hands, rinse them with a lot of water. 3. The pH probe is a delicate instrument. The end of the probe consists of a thin glass bulb. Because this glass is thin, it can break easily. Handle the probe with care and follow your instructor’s directions for cleaning. The pH probe can also dry out easily. Be sure it is kept in a pH 7 buffer solution when not in use. Do not store the probe in water. |
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ACTIVITY 2 |
Principles of
Chemistry Lab
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PURPOSE: |
In this experiment students will learn to: test
organic compound to determine whether they are fats, carbohydrates or
proteins, conduct an experiment to determine how heat and cold effect enzyme
activity, and construct three-dimensional atomic models of organic and
inorganic compounds to observe different types of bonding. |
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SUMMARY: |
In this experiment students will use molecular
model kits to build different types of organic and inorganic compounds found
in living things. Students will also observe the behavior of enzymes when
exposed to heat and cold. Students
will then perform different types of test on organic compounds to determine
whether the compound is a carbohydrate, fat or protein. |
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EQUIPMENT: |
Materials for
this laboratory exercise are set up for lab groups consisting of two to three
students. molecular model kit tweezers 3%
hydrogen peroxide milk 4
test tubes Lugol’s
or idoine solution test
tube rack Benedict’s
solution thermometer raw
egg white 50
mL graduated cylinder raw
chicken or cow liver starch solution |
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SAFETY: |
The teacher should review the following with all students taking part in this laboratory exercise. All chemicals may be disposed of in accordance with your local laws. Review the MSDS sheets of all chemicals used in this experiment. 1. Apron, goggles, and gloves should be worn at all time during lab! 2. Since the students will be using iodine or Lugol’s solution, Benedict’s solution and 3% hydrogen peroxide which are all toxic And caustic, the instructor should review basic safety precautions pertaining to the use of these chemicals. If you should spill
any of these solutions on your hands, rinse them with a lot of water. 3. Since a hot water
bath will be used to denature the enzyme in the liver caution should be observed by students using the heating unit. |
ACTIVITY 3 |
Review of Light
Microscope
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PURPOSE: |
To familiarizes students with the use of a compound light
microscope. Teach students to make
slides of prepared human cells to review wet mount and simple slide staining
techniques. |
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SUMMARY: |
To provide the students with the opportunity to review the parts of a
microscope and proper handling of a compound light microscope. Students will
make slides to review wet mount and simple slide staining techniques. |
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EQUIPMENT: |
Materials for this laboratory exercise are set up for lab groups
consisting of two to three students. water forceps fine
newspaper scissors medicine
dropper slide
and cover slips compound
microscope drawing 3 basic
types of microscope drawing compound
light microscope drawing |
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SAFETY: |
Review basic care and handling of microscopes. No
special safety consideration is required for this activity. |
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ACTIVITY 4 |
Cellular Structure and Function |
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PURPOSE: |
Students observe human cheek cells and their internal
organization. Then they examine the
process of cell division and make drawing of major stages. Students will conduct quantitative
analyses to determine the way in which materials cross cell membranes. |
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SUMMARY: |
Students will make cheek cell slides and observe them under a light
microscope. Students will also
collect data on what type of materials will cross a membrane. Students will use a light microscope to
examine and draw different stages of cell mitosis. |
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EQUIPMENT: |
Materials for this laboratory exercise
are set up for lab groups consisting of two to three students. water scissors forceps ring
stand tooth
picks thistle tube dialysis
tubing 150
mL beakers copper
sulfate 400
mL beakers iodine
solution medicine
dropper wood
charcoal filter
paper funnel 0.1%
silver nitrate 30%
sugar solution methylene
blue stain 50
mL graduated cylinder glass
slide and cover slips potassium
permanganate compound
light microscope starch-sodium
chloride solution prepared
microscope slides of mitosis |
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SAFETY: |
The teacher should review the following with all students taking part in this laboratory exercise. All chemicals may be disposed of in accordance with your local laws. Review the MSDS sheets of all chemicals used in this experiment. 1. Apron should be worn at all time during lab! 2. Review basic care and handling of microscopes 3. Since the students will be using 0.1% silver nitrate, methylene blue stain, and potassium permanganate are all toxic and caustic, the instructor should review basic safety precautions pertaining to the use of these chemicals. If you should spill any of these solutions on your hands, rinse
them
with a lot of water. |
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ACTIVITY 5 |
Extraction of DNA from Cow Thymus |
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PURPOSE: |
Students will extract DNA from a Cow Thymus cells to better understand
the amount of DNA each cell contains. |
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SUMMARY: |
Students first help prepare the cow’s thymus so that the cells in this
organ will begin to swell and break.
Next using meat tenderizer students and alcohol DNA is taken out of
the cells and spun on a pencil |
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EQUIPMENT: |
Materials for this laboratory exercise
are set up for lab groups consisting of two to three students. cheesecloth 100
mL water funnel 250 mL
beaker detergent 95% ethanol ice bath pencil 2 cm piece of calf thymus
(also called sweetbreads) |
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SAFETY: |
The teacher should review the following with all students taking part in this laboratory exercise. All chemicals may be disposed of in accordance with your local laws. Review the MSDS sheets of all chemicals used in this experiment. Apron and goggles should be
worn at all times during this lab! |
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ACTIVITY 6 |
Microscopic Examination of Human Tissue Types |
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PURPOSE: |
Students will examine the structure of the main types of human body
tissue. Students will also identify
the function of the various tissues of the body. |
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SUMMARY: |
Students will use a light microscope to examine, classify, and draw
the thirteen different major tissue types and found in the human body. Students will also identify the function
of the various tissues of the body. |
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EQUIPMENT: |
Materials for
this laboratory exercise are set up for lab groups consisting of two to three
students. Prepared slides of each of the following tissues: Epithelial
tissue Connective/Supportive
Muscle Muscle tissue 1. simple squamous 1. hyaline 1.
striated 2. stratified squamous 2. elastic 2.
smooth 3. cuboidal 3.
blood 3.
cardiac 4. simple
columnar 4. bone 5. stratified columnar 5. adipose |
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SAFETY: |
Review basic care and
handling of microscopes |
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ACTIVITY 7 |
Integumentary
Lab
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PURPOSE: |
Students will examine the appearance and structure
of the skin, hair, scalp, nails, and fingerprints. Students will also collect
data on the sense of touch, including heat, pressure, pain, etc. to observe
the skins role in communication with the environment. |
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SUMMARY: |
Student examine the skin and make comparison of
data collected. |
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EQUIPMENT: |
Materials for this laboratory exercise
are set up for lab groups consisting of two to three students. nails ink
pad ice bath compass metric ruler tweezers microscope stiff brush magnifying glass fingerprint
reference sheet |
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SAFETY: |
Care should be taken not to damage skin nor draw blood with compass
point. |
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ACTIVITY 8 |
Introduction to the Axial Skeletal System
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PURPOSE: |
Students will learn the names, structure, markings, and location of
bones of the axial skeletal system.
Students will also learn internal parts of a long bone. |
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SUMMARY: |
Students will examine and then label the internal structure of a long
bone using real bones and bone models.
Then the students will examine real and plastic models of bones to
learn the names, structure, marking, and location of the bones of the axial
skeleton system. Students will also
classify the bones they examine as being short, flat, long or irregular. |
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EQUIPMENT: |
Materials for this laboratory exercise
are set up for lab groups consisting of two to three students. plastic disarticulating skeleton skulls real articulated skeleton plastic encased
bones of the ear |
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SAFETY: |
Review proper handling
techniques for real bones. |
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ACTIVITY 9 |
Introduction to the Appendicular Skeletal System |
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PURPOSE: |
Students will learn the names, structure, markings, and location of
bones of the appendicular skeletal system.
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SUMMARY: |
Students will examine and then label the internal structure of a long
bone using real bones and bone models.
Then the students will examine real and plastic models of bones to
learn the names, structure, marking, and location of the bones of the axial
skeleton system. Students will also
classify the bones they examine as being short, flat, long or irregular. |
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EQUIPMENT: |
Materials for this laboratory exercise
are set up for lab groups consisting of two to three students. plastic disarticulating skeleton real
articulating skeleton |
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SAFETY: |
Review proper handling techniques for real bones. |
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ACTIVITY 10 |
Introduction to the Muscles
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PURPOSE: |
To teach students the names,
location, origin, and insertions of major muscles of the human body. This will be accomplished by sculpting
muscles onto a plastic human figure. |
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SUMMARY: |
Students will use modeling clay to
sculpt the major muscles of the body onto a plastic human mannequin
figure. Students will learn origin
and insertions of major muscle groups and their primary action. |
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EQUIPMENT: |
Materials for this laboratory exercise
are set up for lab groups consisting of two students working together. modeling clay mannequin
figure reference text |
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SAFETY: |
Review proper handling of mannequin figure, modeling clay, and use of
sculpting instruments. |
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ACTIVITY 11 |
Micro Structure of a Muscle |
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PURPOSE: |
Understand relationship between variables. Develop ability to graph and interpret graphs, find an unknown
variable. |
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SUMMARY: |
Students note changes cold and warm water have on handwriting; bicep
circumference when flexes and relaxed; gastronemius muscle changes when
standing on one’s toes, fatigue effects on finger muscle. |
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EQUIPMENT: |
Materials for this laboratory exercise
are set up for lab groups consisting of two to three students. pan of ice tape strips of paper cloth measure tape tongue depressor string & 500 g weight stopwatch sphygmomanometer |
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SAFETY: |
Watch to make sure that students are not risking injury to their
fingers. |
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ACTIVITY 12 |
Human Reflex Physiology |
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PURPOSE: |
Students will gain a better understanding of how neurons work and
reflex nerve responses. |
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SUMMARY: |
Students test the patellar, Achilles, crossed
extensor, abdominal, plantar, corneal, gag, papillary light, consensual,
ciliospinal, and salivary reflexes. |
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EQUIPMENT: |
Materials for this laboratory exercise
are set up for lab groups consisting of two students. reflex hammer sharp
pencils cot (if available) absorbent cotton tongue depressor metric and 12 in ruler flashlight wide-range
pH paper 100 or 250 mL beaker large bucket w/ fresh 10%
bleach solution disposable autoclave bag wash bottle w/ 10 % bleach
solution |
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SAFETY: |
Properly dispose of materials |
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ACTIVITY 13 |
Dissection of a
Sheep’s Brain
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PURPOSE: |
To learn the names, location, and structures of a mammalian brain. |
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SUMMARY: |
Students will dissect a sheep brain to learn
general anatomy of the mammalian brain. They will familiarize themselves with
the different points of view and plans of section for example dorsal,
ventral, lateral and sagittal, coronal, and horizontal planes of section. |
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EQUIPMENT: |
Materials for this laboratory exercise
are set up for lab groups consisting of two students. sheep’s brain gloves dissecting tray aprons dissecting kit safety goggles |
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SAFETY: |
Preservatives can be dangerous to students that
wear contact lenses. These students,
if possible, should wear eyeglasses during lab. All students must wear gloves and lab aprons during
dissection. Wearing of safety goggles is also strongly advised
during lab. |
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ACTIVITY 14 |
Hearing and Equilibrium Lab; Taste and Olfaction Lab
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PURPOSE: |
To demonstrate knowledge of ear anatomy, test students hearing, and
equilibrium. Students will learn
about the sense of smell through data collecting and analysis. |
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SUMMARY: |
Students will dissect a sheep brain to learn
general anatomy of the mammalian brain. They will familiarize themselves the
different points of view and plans of section for example dorsal, ventral,
lateral and sagittal, coronal, and horizontal planes of section. |
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EQUIPMENT: |
Materials for this laboratory exercise
are set up for lab groups consisting of two to three students. chalk Q-tips cotton almond toothpicks distilled water oranges ticking
watch paper towel paper cups sugar crystal tuning
forks peppermint rotating stool acetic acid solution vial of NaCl
solution
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SAFETY: |
Equilibrium subjects should not be easily inclined
to dizziness on rotation or turning movements; rotation should be stopped if
subject becomes nauseous; classmates should be prepared to catch, hold o9r
support subject(s).
A 10% bleach solution should be used on desk and
counter tops in the classroom before beginning the smell lab. For the sense of smell lab it is important that the students not share
anything in this part of the lab.
This includes cups and anything that they have in their mouths. Students should dispose of their own waste
materials. |
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ACTIVITY 15 |
Dissection of a Cow’s Eye |
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PURPOSE: |
Students will dissect a cow’s eye to learn general
anatomy of the mammalian eye. They will familiarize themselves with the
different points of view and plans of section for example dorsal, ventral,
lateral and sagittal, coronal, and horizontal planes of section. |
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SUMMARY: |
In groups of two students will dissect and examine the different parts
of a cow’s eye. |
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EQUIPMENT: |
Materials for this laboratory exercise
are set up for lab groups consisting of two students. cow’s eye gloves dissecting tray aprons dissecting
kit safety goggles |
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SAFETY: |
Preservatives can be dangerous to students that
wear contact lenses. These students,
if possible, should wear eyeglasses during lab. All students must wear gloves and lab aprons during
dissection. Wearing of safety goggles
is also strongly advised during lab. |
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ACTIVITY 16 |
Lab on Human Vision |
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PURPOSE: |
Determine near point vision, visual acuity, accommodation,
astigmatism, and visual mapping. To
gain understanding of human vision. |
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EQUIPMENT: |
Materials for this laboratory exercise
are set up for lab groups consisting of two to three students. pins cm scale astigmatism chart index cards visual map peripheral vision cards Snellen chart |
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SAFETY: |
None |
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ACTIVITY 17 |
Forensic Science – Caught By A Kiss |
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PURPOSE: |
This lab is intended to familiarize students with the activity of the
enzyme amylase, the primary digestive component of human saliva. It will also emphasize to students the
value of finding saliva at a crime scene, because helpful DNA evidence can be
isolated from saliva. |
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SUMMARY: |
This lab allows students to work together using forensic science
techniques to determine which of 8 suspects was actually at the scene of the crime. |
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EQUIPMENT: |
Materials for this laboratory exercise
are set up for lab groups consisting of two to three students. 8 toothpicks 8 straws 8 paper cups 24
petri plates 8 envelopes 24 resealable
bags 50 absorbent cotton balls labels 500 mL bottle of
Melt-N-Pour starch agar 30mL IKI potassium iodide
solution |
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SAFETY: |
Prudent lab safety practices should be followed at all times during
this lab. One portion of the lab
requires use of boiling hot liquids. Students should be warned of the danger
of getting burned. |