Biology II AP Core Labs

   Lab 11 - Enzymes in Cells

Links:


Biology II AP Curriculum Page

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Purpose:
Student will learn the effects that changes in temperature, pH and concentration have on the activity of an enzyme. These tasks will be accomplished during a hands on laboratory investigation in which the students will collect data, graph their results and use the information they obtained to answer questions.

Objectives Links:
Introduction to Metabolism

Process Skills Checklist

Materials:
Per lab group (3-4 students):

  • sample of ground potato pulp (about 10 mL)
  • four water baths: ice
  • room temperature
  • body temp.
  • boiling water
  • 5 test tubes (13 x 100 mm)
  • fresh 3% hydrogen peroxide (20 25 mL)
  • potatoes
  • metric ruler
  • thermometer
  • several paper towels
  • calculator
  • marking pencil and labels for marking test tubes
  • watch
  • ice
  • hot plate
  • blender
  • cheese cloth

Safety Concerns:

  1. Instruct students to wear a lab apron and their safety goggles or glasses at all times during this laboratory exercise.
  2. Remind students that they should perform only the procedures described in the student directions and only under the supervision of the teacher.
  3. Remind students that should an accident occur during this laboratory exercise, the student should stop work immediately and notify the teacher.

Revision Date:
Summer 2001

ISD 30 Science Dept. Bartlesville Independent School District 30
Science Department

Biology II AP Curriculum