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Purpose: Student will learn the effects that changes in temperature, pH and concentration have on the activity of an enzyme. These tasks will be accomplished during a hands on laboratory investigation in which the students will collect data, graph their results and use the information they obtained to answer questions.
Objectives Links:
Introduction to Metabolism
Process Skills Checklist
Materials:
Per lab group (3-4 students):
- sample of ground potato pulp (about 10 mL)
- four water baths: ice
- room temperature
- body temp.
- boiling water
- 5 test tubes (13 x 100 mm)
- fresh 3% hydrogen peroxide (20 25 mL)
- potatoes
- metric ruler
- thermometer
- several paper towels
- calculator
- marking pencil and labels for marking test tubes
- watch
- ice
- hot plate
- blender
- cheese cloth
Safety Concerns:
- Instruct students to wear a lab apron and their safety goggles or glasses at all times during this laboratory exercise.
- Remind students that they should perform only the procedures described in the student directions and only under the supervision of the teacher.
- Remind students that should an accident occur during this laboratory exercise, the student should stop work immediately and notify the teacher.
Revision Date: Summer 2001
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