Biology II AP Core Labs

   Lab 7 - Formation of Proteinoid Microspheres & Coacervates

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Purpose:
This activity will allow each student to make and observe proteinoid microspheres and coacervates. The characteristics of these cell-like spheres will allow students to develop an understanding of living cell properties. Students will determine if any of these entities possess the characteristics of life.

Objectives Links:
Structure & Function of Prokaryotic, Eukaryotic & Subcellular Organizations

Process Skills Checklist

Materials:

  • Bunsen burner
  • distilled water
  • eye droppers
  • 0.1 M HCl (add 3.65 mL 1N HCL to 1 liter distilled water)
  • 3 test tubes
  • 1% gelatin sol'n (add 1 g gelatin to 100 mL dist. water)
  • test tube stopper
  • 1% gum arabic sol'n (add 1 g to 100 mL distilled water)
  • microscope
  • 1 g glutamic acid
  • microscope slides
  • 1 g aspartic acid
  • cover slips
  • 2 g of mixed amino acids
  • compound microscope
  • saline solution
  • dilute dye (i.e. congo red, methylene blue, neutral red)

Safety Concerns:
Basic chemical handling safety; wear safety glasses or goggles at all times; wear aprons.

Revision Date:
Summer 2001

ISD 30 Science Dept. Bartlesville Independent School District 30
Science Department

Biology II AP Curriculum