|
Links:
Honors Biology I Curriculum Page
Previous Core Lab
Next Core Lab
|
|
Purpose:
To use specific tests to identify monosaccharides, disaccharides, polysaccharides, proteins, and lipids.
To use the results to identify three unknown substances.
Summary:
Students will test a monosaccharide, disaccharide, and polysaccharide using the iodine and Benedict's tests. They will test for protein using Biuret reagent, and will test for lipid using Sudan III. They will use the results of these tests to identify 3 different unknown substances.
Objectives Links:
5. Matter, Energy, and Organization in Living Systems
B: Matter and Energy Cycles
Process Skills Checklist
Materials:
- Test tubes
- Test tube racks
- Boiling water bath
- Beakers
- Iodine
- Droppers
- Benedict's solution
- Biuret reagent
- Sudan III
- Samples of the known substances (potato, apple, glucose solution, gelatin solution, vegetable oil)
- Various organic substances to use as unknowns
Safety Concerns:
Do not eat or drink. Be careful with glassware. Report all spills. Use caution with hot materials. Wear safety goggles during lab.
Revision Date:
July 2007
|
